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人黑色素瘤細胞-ATCC細胞庫代理
ATCC CRL-3226 VMM5A
| 基本信息 | |
| 資源編號 | ATCC CRL-3226 |
| 資源名稱 | VMM5A |
| 種屬 | 人黑色素瘤細胞 |
| 類型 | Melanocyte |
| 形態 | Epithelial-like |
| 提供形式 | 凍存管 |
| 致病類型 | Melanoma, Stage IIIC; malignant |
| 安全等級 | 1 |
| 模式菌株 | 未知 |
| 應用領域 | Drug screening Development of targeted therapy Development of combination therapy Tumor vaccine development |
| 說明書 | 點擊下載 |
| 產品圖片 | 點擊下載 |
| 培養方法 | |
| 培養基 | 90%RPMI-1640+10%FBS |
| 傳代方法 | Volumes are for a T-75 flask; Adjust accordingly Remove and discard the cell culture medium from the flask. Rinse the cell monolayer with Dulbecco’s PBS without calcium or magnesium and remove. Add 3 to 4 ml of the trypsin-EDTA solution, rotate flask to rinse cell monolayer, remove trypsin solution, and incubate at 37oC. Once the cells appear to be detached, add 10 ml of complete growth medium with a pipette to the cell suspension to inactivate the trypsin. Gently wash any remaining cells from the growth surface of the flask. Check the cells with the microscope to be sure that most (>95%) are single cells. If cell clusters are apparent, continue to disperse the cells with gentle pipetting. Subculture as necessary. Place the flask back into the incubator. Examine the culture the following day to ensure the cells have reattached and are actively growing. Repeat when cells reach confluence. |
| 生長條件 | 95%空氣+5%二氧化碳37攝氏度 |
| 生長特性 | 貼壁生長 |
| 存儲條件 | 50% RPMI-1640+40%FBS+10%DMSO液氮 |
| 詳細說明 | |
| Age | 81 |
| Gender | Male |
| Ethnicity | Caucasian |
| Derivation | Derived from tumors taken from tumor-involved lymph nodes from patients at the University of Virginia |
| Clinical Data | Primary Site Right Posterior Shoulder; Metastatic Site Lymph Node, Right Neck |
| Antigen Expression | Positives High VEGF-R2, Low GP100, Tyrosinase, Low MAGE-A1, MMP-1 |
| HLA Typing | A2.1,A1101,B39,B44, BW4,BW6,C7(17)DR7, DR8,DR11,DQ2,DQ7 |
| STR Profile | Amelogenin X,Y D5S818 9 D7S820 9,10 D13S317 12 D16S539 12 vWA 16 TH01 9.3 TPOX 8,11 CSF1PO 11 |
| Sterility Tests | Pass |
| Passage History | Unknown. 2 passages from 2003 frozen cell line stock, but it is not known how long the line was in culture after being established from tumor tissue obtained in 1992. |
| Year of Origin | 1992 |
| 描述 | NRAS: wt CDKN2A: wt BRAF Mutation: V600E APC Mutation PDGFRA Mutation: D842V PTEN Mutation: R173H more sensitive to BAY43-9006 (BRAF kinase inhibitor) and to rapamycin (mTOR kinase inhibitor), compared to cell lines with wild-type B-Raf |
人黑色素瘤細胞-ATCC細胞庫代理
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